The Tigers lost their first replay review in the fifth inning.

Nick Markakis hit a slow-roller to Ian Kinsler at second base, but Tigers shortstop Andrew Romine appeared to drop the ball when he tried to transfer it from his glove after stepping on second.

Second-base umpire Kerwin Danley ruled the lead runner, Steve Lombardozzi, safe, and manager Brad Ausmus challenged the play.

Two minutes later, after review in New York, the call was upheld.

It was ruled that Romine had an "involuntary release" of the ball, an MLB source said.

Detroit won its first two challenges in Wednesday's game.

Here's the video from Friday's challenge: